Enterococcus Confirmatory HiVeg Broth with Penicillin

[所属分类:培养基配方] [发布时间:2020-7-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Enterococcus Confirmatory HiVeg Broth
with Penicillin

培养基配方
Composition per liter:
NaCl.........................................................................65.0g
Glucose ......................................................................5.0g
Plant hydrolysate.............................................................5.0g
Yeast extract.................................................................5.0g
NaN3 .........................................................................0.4g
Methylene Blue...............................................................0.01g
Penicillin solution.........................................................10.0mL
pH 8.0 ± 0.2 at 25°C
Source: This medium, without penicillin, is available as a premixed
powder from HiMedia.
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Penicillin Solution:
Composition per 10.0mL:
Penicillin.....................................................................650U
Preparation of Penicillin Solution: Add penicillin to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except penicillin solu-
tion, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL sterile pen-
icillin solution. Mix thoroughly.
Use: For the cultivation of entercocci in water, sewage, and feces.