Enterococcus Agar (m-Enterococcus Agar) (Azide Agar)

[所属分类:培养基配方] [发布时间:2020-7-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Enterococcus Agar
(m-Enterococcus Agar)
(Azide Agar)

培养基配方
Composition per liter:
Pancreatic digest of casein.................................................15.0g
Agar........................................................................10.0g
Papaic digest of soybean meal................................................5.0g
Yeast extract................................................................5.0g
KH2PO4 ......................................................................4.0g
Glucose......................................................................2.0g
NaN3 ........................................................................0.4g
Triphenyltetrazolium chloride ...............................................0.1g
pH 7.2 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Cool to 45°–50°C. Do not autoclave. Pour into sterile Petri
dishes.
Use: For the isolation, cultivation, and enumeration of entercocci in
water, sewage, and feces by the membrane filter method. For the direct
plating of specimens for the detection and enumeration of fecal strep-
tococci.