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Enterococcosel™ Broth

[所属分类:培养基配方] [发布时间:2020-7-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Enterococcosel™ Broth

培养基配方
Composition per liter:
Pancreatic digest of casein...................................................17.0g
Oxgall .......................................................................10.0g
NaCl...........................................................................5.0g
Yeast extract..................................................................5.0g
Peptic digest of animal tissue ................................................3.0g
Esculin........................................................................1.0g
Sodium citrate.................................................................1.0g
Ferric ammonium citrate........................................................0.5g
NaN3 .........................................................................0.25g
pH 7.1 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring until dissolved. Distribute into tubes or flasks. Autoclave for 15
min at 15 psi pressure–121°C.
Use: For the cultivation and differentiation of group D streptococci
(enterococci).

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