Enterococcosel™ Agar

[所属分类:培养基配方] [发布时间:2020-7-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Enterococcosel™ Agar

培养基配方
Composition per liter:
Pancreatic digest of casein..................................................17.0g
Agar ........................................................................13.5g
Oxgall.......................................................................10.0g
NaCl.........................................................................5.0g
Yeast extract................................................................5.0g
Peptic digest of animal tissue...............................................3.0g
Esculin .....................................................................1.0g
Sodium citrate...............................................................1.0g
Ferric ammonium citrate......................................................0.5g
NaN3 .......................................................................0.25g
pH 7.1 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring and bring to boiling. Distribute into tubes or flasks. Autoclave
for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or
leave in tubes.
Use: For the rapid, selective isolation, cultivation, and enumeration of
fecal group D streptococci (enterococci). For the cultivation of staphy-
lococci and Listeria monocytogenes.