Enterococci Presumptive Broth

[所属分类:培养基配方] [发布时间:2020-7-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Enterococci Presumptive Broth

培养基配方
Composition per liter:
Glucose .....................................................................5.0g
Pancreatic digest of casein..................................................5.0g
Yeast extract................................................................5.0g
NaN3 ........................................................................0.4g
Bromthymol Blue ..........................................................32.0mg
pH 8.4 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the isolation and identification of enterococci from water by
the presumptive test. Bacteria that produce acid and turn the medium
yellow and turbid after incubation at 45°C are presumptive entero-
cocci.