Enterococci Confirmatory Broth

[所属分类:培养基配方] [发布时间:2020-7-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Enterococci Confirmatory Broth

培养基配方
Composition per liter:
NaCl..........................................................................65.0g
Glucose.......................................................................5.0g
Pancreatic digest of casein...................................................5.0g
Yeast extract.................................................................5.0g
NaN3 .........................................................................0.4g
Methylene Blue...............................................................10.0mg
Penicillin...................................................................650U
pH 8.0 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Preparation of Medium: Add components, except penicillin, to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 25°C. Aseptically add penicillin. Mix thoroughly.
Use: For the identification of enterococci from water by the confirma-
tory test.