Enterococci Confirmatory Agar

[所属分类:培养基配方] [发布时间:2020-7-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Enterococci Confirmatory Agar

培养基配方
Composition per liter:
Agar.....................................................................15.0g
Glucose...................................................................5.0g
Pancreatic digest of casein...............................................5.0g
Yeast extract.............................................................5.0g
NaN3 .....................................................................0.4g
Methylene Blue..........................................................10.0mg
Enterococci confirmatory broth........................................variable
pH 8.0 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes. Autoclave for 15 min at 15 psi pres-
sure–121°C. Allow tubes to cool in a slanted position. Add sufficient
amount of Enterococci confirmatory broth (see below) to cover half the
slant.
Use: For the identification of enterococci from water by the confirma-
tory test.