Entamoeba Medium (Endamoeba Medium)

[所属分类:培养基配方] [发布时间:2020-7-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Entamoeba Medium
(Endamoeba Medium)

培养基配方
Composition per liter:
Liver infusion..............................................................272.0g
Rice powder..................................................................14.2g
Agar.........................................................................11.0g
Proteose peptone..............................................................5.5g
Sodium glycerophosphate.......................................................3.0g
NaCl..........................................................................2.7g
Horse serum.................................................................50.0mL
pH 7.0 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Rice Powder:
Composition per 15.0g:
Rice powder...................................................................15.0g
Preparation of Rice Powder:  Sterilize rice powder at 160°C for 60
min. Do not overheat or rice powder will scorch.
Preparation of Medium: Add components, except horse serum
and rice powder, to distilled/deionized water and bring volume to
994.0mL. Mix thoroughly. Gently heat and bring to boiling. Distribute
into tubes in 7.0mL volumes. Autoclave for 15 min at 15 psi pressure–
121°C. Allow tubes to cool in a slanted position. Aseptically add
enough sterile horse serum to each tube to cover about half the slant.
Aseptically add 0.1g of sterile rice powder to each tube.
Use: For the cultivation of Entamoeba histolytica.