Enteric Fermentation Base (Fermentation Base for Campylobacter)

[所属分类:培养基配方] [发布时间:2020-7-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Enteric Fermentation Base
(Fermentation Base for Campylobacter)

培养基配方
Composition per liter:
Peptic digest of animal tissue .........................................10.0g
NaCl.....................................................................5.0g
Beef extract.............................................................3.0g
Carbohydrate solution.................................................100.0mL
Andrade’s indicator...................................................10.0mL
pH 7.2 ± 0.1 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Carbohydrate Solution:
Composition per 100.0mL:
Carbohydrate............................................................10.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Filter sterilize. Glucose, lactose, mannitol, sucrose, adonitol, arabi-
nose, cellobiose, dulcitol, glycerol, inositol, salicin, xylose, or other
carbohydrates may be used. For the preparation of expensive carbohy-
drate solutions (adonitol, arabinose, cellobiose, dulcitol, glycerol, inos-
itol, salicin, or xylose), 5.0g of carbohydrate  per 100.0mL of distilled/
deionized water may be used.
Andrade’s Indicator:
Composition per 100.0mL:
NaOH (1N solution)......................................................16.0mL
Acid Fuchsin.............................................................0.1g
Caution: Acid Fuchsin is a potential carcinogen and care must be tak-
en to avoid inhalation of the powdered dye and contact with the skin.
Preparation of Andrade’s Indicator:  Add components to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Add components, except carbohydrate
solution, to distilled/deionized water and bring volume to 900.0mL.
Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes
that contain an inverted Durham tube in 9.0mL volumes. Autoclave for
15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 1.0mL
of sterile carbohydrate solution per tube. Mix thoroughly.
Use: For the cultivation and differentiation of a variety of bacteria
based on their ability to ferment different carbohydrates. Bacteria that
produce acid from carbohydrate fermentation turn the medium dark
pink to red. Bacteria that produce gas have a bubble trapped in the Dur-
ham tube.