Entamoeba HiVeg Medium

[所属分类:培养基配方] [发布时间:2020-7-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Entamoeba HiVeg Medium

培养基配方
Composition per liter:
Agar .......................................................................11.0g
Plant infusion No.1.........................................................10.8g
Plant peptone No.3...........................................................5.5g
Sodium glycerophosphate......................................................3.0g
NaCl.........................................................................2.7g
pH 7.0 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Allow the tubes to solidify in a slanted position.
Cover about half of the slant with fresh sterile horse seum saline mix-
ture (1:6) and add a 5mm loopful of rice powder that has been sterilized
in an oven at 160°C. Scorching should be prevented.
Use: For the cultivation of Entamoeba histolytica.