Cornmeal Agar with Strep100 and Tet100 (ATCC Medium 2285)

[所属分类:培养基配方] [发布时间:2020-5-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cornmeal Agar with Strep100 and Tet100
(ATCC Medium 2285)

培养基配方
Composition per liter:
Agar ........................................................................15.0g
Cornmeal, solids from infusion ...............................................2.0g
Antibiotic solution ........................................................10.0mL
pH 5.6–6.0 at 25°C
Source: This medium without antibiotics is available as a premixed
powder from BD Diagnostic Systems and Oxoid Unipath.
Preparation of Medium: Add components except antibiotic solution
to 990.0mL distilled/deionized water. Mix thoroughly. Gently heat until
boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 45–50°C. Aseptically add 10.0mL sterile antibiotic
solution. Mix thoroughly. Pour into sterile Petri dishes or leave in tubes.
Antibiotic Solution:
Composition per 10.0mL:
Tetracycline...................................................................0.1g
Streptomycin sulfate...........................................................0.1g
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Use: For the cultivation and maintenance of fungi.