Cornmeal Agar with Soil Extract

[所属分类:培养基配方] [发布时间:2020-5-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cornmeal Agar with Soil Extract

培养基配方
Composition per liter:
Cornmeal.....................................................................50.0g
Agar .........................................................................7.5g
Soil extract................................................................50.0mL
Soil Extract:
Composition per 200.0mL:
African Violet soil..........................................................77.0g
Na2CO3 .......................................................................0.2g
Preparation of Soil Extract: Add components to 200.0mL of dis-
tilled/deionized water. Mix thoroughly. Autoclave for 60 min at 15 psi
pressure–121°C. Filter through paper and reserve filtrate.
Preparation of Medium: Add cornmeal to distilled/deionized wa-
ter and bring volume to 800.0mL. Leave overnight in refrigerator. Heat
to 60°C for 1 hr. Add 50.0mL of soil extract. Bring volume to 1.0L with
distilled/deionized water. Add agar. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Helicodendron tubulo-
sum, Microsporum distortum, Mortierella humilis, Mortierella hygro-
phila, Mortierella minutissima, and Nigrospora sphaerica.