Cooked Meat Medium, Modified

[所属分类:培养基配方] [发布时间:2020-5-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cooked Meat Medium, Modified

培养基配方
Composition per liter:
Cooked meat medium..............................................................66.0g
Solution A.......................................................................1.0L
pH 6.8 ± 0.2 at 25°C
Cooked Meat Medium:
Composition per 481g :
Beef heart.....................................................................454.0g
Proteose peptone................................................................20.0g
NaCl.............................................................................5.0g
Glucose..........................................................................2.0g
Source: Cooked meat medium is available in dehydrated form from
BD Diagnostic Systems.
Solution A:
Composition per liter:
Pancreatic digest of casein.....................................................10.0g
Glucose..........................................................................2.0g
Soluble starch...................................................................1.0g
Sodium thioglycolate.............................................................1.0g
Neutral Red (1% aqueous)........................................................5.0mL
Preparation of Solution A: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat until dis-
solved.
Preparation of Medium:  Add 1.0g of dehydrated cooked meat medi-
um to each of 66 test tubes. Add 15.0mL of solution A to each test tube.
Allow meat particles to rehydrate. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the cultivation of a variety of anaerobic microorganisms.