Cooked Meat Medium, Modified (BAM M43)

[所属分类:培养基配方] [发布时间:2020-5-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cooked Meat Medium, Modified
(BAM M43)

培养基配方
Composition per tube:
Cooked meat medium........................................................1.0g
Diluent ..................................................................1.0L
pH 6.8 ± 0.2 at 25°C
Cooked Meat Medium:
Composition per 481g :
Beef heart.............................................................454.0g
Proteose peptone........................................................20.0g
NaCl.....................................................................5.0g
Glucose..................................................................2.0g
Source: Cooked meat medium is available in dehydrated form from
BD Diagnostic Systems.
Diluent:
Composition per liter:
Pancreatic digest of casein.............................................10.0g
Glucose..................................................................2.0g
Soluble starch...........................................................1.0g
Sodium thioglycolate.....................................................1.0g
Neutral Red (1% aqueous)................................................5.0mL
Preparation of Diluent: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat until dis-
solved.
Preparation of Medium: Add 1.0g of dehydrated cooked meat
medium and 15.0mL diluent to 20 × 150mm test tubes. Let meat parti-
cles rehydrate. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C.