Cooked Meat Medium with Peptone and Yeast Extract

[所属分类:培养基配方] [发布时间:2020-5-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cooked Meat Medium with
Peptone and Yeast Extract

培养基配方
Composition per liter:
Heart muscle.................................................................454.0g
Peptone.......................................................................40.0g
Beef extract..................................................................10.0g
NaCl...........................................................................5.0g
Yeast extract..................................................................5.0g
Glucose .......................................................................2.0g
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Finely chop beef heart. Add approximately
1.5g of heart particles to test tubes. Add remaining components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Dis-
tribute into tubes in 10.0mL volumes. Autoclave for 15 min at 15 psi
pressure–121°C. Slowly cool tubes to prevent expulsion of meat parti-
cles.
Use: For the cultivation and maintenance of Peptostreptococcus mag-
nus.