CLED Agar with Andrade’s Indicator (Cystine Lactose Electrolyte Deficient Agar with Andrade’s Indicator)

[所属分类:培养基配方] [发布时间:2020-5-6] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CLED Agar with Andrade’s Indicator
(Cystine Lactose Electrolyte Deficient Agar
with Andrade’s Indicator)

培养基配方
Composition per liter:
Agar .........................................................................................15.0g
Pancreatic digest of casein..........................................................10.0g
Peptone.......................................................................................4.0g
Beef extract.................................................................................3.0g
L -Cystine................................................................................0.128g
Bromthymol Blue.......................................................................0.02g
Andrade’s indicator..................................................................10.0mL
pH 7.5 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Oxoid
Unipath.
Caution:  Acid Fuchsin is a potential carcinogen and care must be tak-
en to avoid inhalation of the powdered dye and contamination of the
skin.
Andrade’s Indicator:
Composition per 100.0mL:
NaOH (1N solution).................................................................16.0mL
Acid Fuchsin...............................................................................0.1g
Preparation of Andrade’s Indicator: Add Acid Fuchsin to
NaOH solution and bring volume to 100.0mL with distilled/deionized
water.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°–55°C. Pour into sterile Petri dishes or distribute
into sterile tubes.
Use: For the differentiation of microorganisms based on colony char-
acteristics.