CLED HiVeg Agar with Andrade’s Indicator

[所属分类:培养基配方] [发布时间:2020-5-6] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CLED HiVeg Agar with Andrade’s Indicator

培养基配方
Composition per liter:
Agar......................................................................15.0g
Lactose...................................................................10.0g
Plant hydrolysate .........................................................4.0g
Plant peptone .............................................................4.0g
Plant extract..............................................................3.0g
L -Cystine...............................................................0.128g
Andrade’s indicator.......................................................0.1g
Bromo Thymol Blue.........................................................0.02g
pH 7.5 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Caution:  Acid Fuchsin in Andrade indicator is a potential carcinogen
and care must be taken to avoid inhalation of the powdered dye and
contamination of the skin.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°–55°C. Pour into sterile Petri dishes or distribute
into sterile tubes.
Use: For the isolation and differentiation of urinary pathogens on the
basis of lactose fermentation.