Clausen Medium (Dithionite Thioglycolate, HS T, Broth)

[所属分类:培养基配方] [发布时间:2020-5-6] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clausen Medium
(Dithionite Thioglycolate, HS T, Broth)

培养基配方
Composition per liter:
Pancreatic digest of casein................................................15.0g
Glucose.....................................................................6.0g
Yeast extract...............................................................6.0g
Glycerol ...................................................................5.0g
Papaic digest of soybean meal...............................................3.0g
Tween™ 80...................................................................3.0g
NaCl........................................................................2.5g
K2HPO4 .....................................................................2.0g
L- Asparagine..............................................................1.25g
Sodium citrate..............................................................1.0g
Agar.......................................................................0.75g
L- Cysteine ................................................................0.5g
Sodium thioglycolate........................................................0.5g
MgSO4 ·7H2O................................................................0.4g
Sodium dithionite...........................................................0.4g
Lecithin....................................................................0.3g
CaCl2 ·2H2O ..............................................................4.0mg
MnCl2 ·4H2O ..............................................................2.0mg
CoSO4 ·7H2O...............................................................1.0mg
CuSO4 ·5H2O...............................................................1.0mg
FeSO4 ·7H2O...............................................................1.0mg
ZnSO4 ·7H2O...............................................................1.0mg
Resazurin..................................................................1.0mg
pH 7.1 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Oxoid
Unipath.
Preparation of Medium: Add Tween™ 80 and glycerol to dis-
tilled/deionized water and bring volume to 1.0L. Add remaining com-
ponents. Gently heat and bring to boiling. Distribute into tubes or
flasks. Autoclave for 15 min at 15 psi pressure–121°C. The medium
must not be resterilized.
Use:  For sterility testing by the membrane filter method or the tube
dilution method to determine the presence of microbial contamination
in a variety of specimens.