Clausen Medium

[所属分类:培养基配方] [发布时间:2020-5-6] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Clausen Medium

培养基配方
Composition per liter:
Casein enzymatic hydrolysate ..............................................15.0g
Glucose ....................................................................6.0g
Yeast extract...............................................................6.0g
Papaic digest of soybean meal...............................................3.0g
NaCl........................................................................2.5g
K2HPO4 .....................................................................2.0g
L -Asparagine..............................................................1.25g
Sodium citrate..............................................................1.0g
Agar ......................................................................0.75g
Na-thioglycollate ..........................................................0.5g
L -Cystine..................................................................0.5g
MgSO4 ......................................................................0.4g
Sodium dithionate...........................................................0.4g
Lecithin....................................................................0.3g
CaCl2 .....................................................................4.0mg
MnCl2 .....................................................................2.0mg
CoSO4 .....................................................................1.0mg
CuSO4 .....................................................................1.0mg
FeSO4 .....................................................................1.0mg
Resazurin .................................................................1.0mg
ZnSO4 .....................................................................1.0mg
Glycerol ..................................................................5.0mL
Polysorbate 80.............................................................3.0mL
pH 7.1 ± 0.2 at 25°C
Source: This medium, without glycerol or polysorbate 80, is available
as a premixed powder from HiMedia.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat to boil-
ing. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile
Petri dishes or leave in tubes. The medium must not be resterilized.
Use:  For sterility testing by the membrane filter method or the tube
dilution method to determine the presence of microbial contamination
in a variety of specimens.