Citrobacter diversus Medium

[所属分类:培养基配方] [发布时间:2020-5-6] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Citrobacter diversus Medium

培养基配方
Composition per liter:
Component 1............................................................500.0mL
Component 2............................................................250.0mL
Component 3............................................................250.0mL
Component 1:
Composition per 500.0mL:
Agar .....................................................................7.5g
Pancreatic digest of casein...............................................7.5g
Papaic digest of soybean meal.............................................2.5g
Preparation of Component 1: Add components to distilled/deion-
ized water and bring volume to 500.0mL. Mix thoroughly. Gently heat
and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 45°–50°C.
Component 2:
Composition per 250.0mL:
Agar ......................................................................7.5g
Pancreatic digest of casein................................................7.5g
Papaic digest of soybean meal..............................................2.5g
L- Tyrosine................................................................1.0g
Preparation of Component 2: Add components to distilled/deion-
ized water and bring volume to 250.0mL. Mix thoroughly. Gently heat
and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 45°–50°C.
Component 3:
Composition per 250.0mL:
L- Tyrosine.................................................................1.0g
Preparation of Component 3: Add components to distilled/deion-
ized water and bring volume to 250.0mL. Mix thoroughly. Gently heat
and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C.
Rapidly cool in ice water (0°C) for 20 min. A fine, white crystalline
precipitate should form.
Preparation of Medium: Pour sterile component 1 into sterile Petri
dishes in 18.0mL volumes. Allow agar to solidify. Warm component 3
to 50°C for 20 min. Aseptically combine component 2 and component
3. Mix thoroughly. Pour 7.0mL of mixture over the solidified compo-
nent 1 agar.
Use: For the isolation and cultivation of Citrobacter diversus.