Citrate Medium, Koser’s Modified

[所属分类:培养基配方] [发布时间:2020-5-6] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Citrate Phosphate Buffered Glucose Medium

培养基配方
Composition per liter:
Solution A..............................................................750.0mL
Solution C..............................................................200.0mL
Solution B...............................................................50.0mL
pH 3.5 ± 0.2 at 25°C
Solution A:
Composition per 750.0mL:
(NH4)2SO4 ................................................................3.0g
Citric acid, anhydrous...................................................1.92g
Na2HPO4 .................................................................1.23g
MgSO4 ·7H2O..............................................................0.5g
KCl.......................................................................0.1g
Ca(NO3)2 ·4H2O .........................................................0.02g
FeSO4 ·7H2O.............................................................0.01g
Preparation of Solution A: Add components to distilled/deionized
water and bring volume to 750.0mL. Mix thoroughly.
Solution B:
Composition per 50.0mL:
Glucose ................................................................10.0g
Yeast extract............................................................1.0g
Preparation of Solution B: Add components to distilled/deionized
water and bring volume to 50.0mL. Mix thoroughly.
Solution C:
Composition per 200.0mL:
Agarose (electrophoresis grade) .........................................6.0g
Preparation of Solution C: Add agarose to distilled/deionized wa-
ter and bring volume to 200.0mL. Mix thoroughly.
Preparation of Medium: Prepare solutions A, B, and C. Autoclave
solutions separately for 15 min at 15 psi pressure–121°C. Cool to 50°–
55°C. Combine solutions A, B, and C. Mix thoroughly. Immediately
distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Acidiphilium organovo-
rum.