CK1 Medium

[所属分类:培养基配方] [发布时间:2020-5-6] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CK1 Medium

培养基配方
Composition per liter:
MgSO4 ·7H2O................................................................3.0g
KNO3 .......................................................................2.0g
CaCl2 ......................................................................1.4g
Ferric citrate.............................................................0.02g
Glucose solution.........................................................100.0mL
K2HPO4 solution...........................................................10.0mL
Glucose Solution:
Composition per 100.0mL:
D- Glucose................................................................10.0g
Preparation of Glucose Solution: Add  D- glucose to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
K2 HPO4 Solution:
Composition per 10.0mL:
K2HPO4 ...................................................................2.5mg
Preparation of K2HPO4 Solution: Add K2HPO4 to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Preparation of Medium: Add components, except glucose solution
and K2HPO4 solution, to distilled/deionized water and bring volume to
890.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 25°C. Aseptically add sterile glucose solution and
K2HPO4 solution. Mix thoroughly. Aseptically distribute into sterile
tubes or flasks.
Use: For the cultivation of myxobacteria.