Cinnamate Medium

[所属分类:培养基配方] [发布时间:2020-4-30] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cinnamate Medium

培养基配方
Composition per liter:
NaHCO3 .....................................................................2.5g
MgCl2 ·6H2O ..............................................................2.03g
Cinnamic acid..............................................................1.48g
KH2PO4 ....................................................................1.36g
NH4Cl .....................................................................0.53g
Na2S·9H2O.................................................................0.24g
CaCl2 ·2H2O ..............................................................0.15g
Yeast extract..............................................................0.05g
Modified Wolfe’s metals..................................................10.0mL
Wolfe’s vitamin solution..................................................10.0mL
pH 7.5–7.7 at 25°C
Modified Wolfe’s Metals:
Composition per liter:
Na2SeO3 ...................................................................10.0mg
NaWO4 ·2H2O ..............................................................10.0mg
NiCl2 ·6H2O...............................................................10.0mg
Wolfe’s metals solution.....................................................1.0L
Preparation of Modified Wolfe’s Metals: Combine the compo-
nents. Mix thoroughly.
Wolfe’s Metals Solution:
Composition per liter:
MgSO4 ·7H2O.................................................................3.0g
Nitrilotriacetic acid........................................................1.5g
NaCl.........................................................................1.0g
MnSO4 ·H2O..................................................................0.5g
CaCl2 .......................................................................0.1g
CoCl2 ·6H2O ................................................................0.1g
FeSO4 ·7H2O.................................................................0.1g
ZnSO4 ·7H2O.................................................................0.1g
AlK(SO4)2 ·12H2O...........................................................0.01g
CuSO4 ·5H2O................................................................0.01g
H3BO3 ......................................................................0.01g
Na2MoO4 ·2H2O .............................................................0.01g
Preparation of Wolfe’s Metals Solution: Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with
KOH. Add distilled/deionized water to 1.0L. Add remaining compo-
nents. Mix thoroughly.
Wolfe’s Vitamin Solution:
Composition per liter:
Pyridoxine·HCl...............................................................0.01g
p-Aminobenzoic acid...........................................................5.0mg
Calcium pantothenate .........................................................5.0mg
Nicotinic acid................................................................5.0mg
Riboflavin ...................................................................5.0mg
Thiamine·HCl ................................................................5.0mg
Thioctic acid ................................................................5.0mg
Biotin .......................................................................2.0mg
Folic acid....................................................................2.0mg
Cyanocobalamin ............................................................100.0μg
Preparation of Wolfe’s Vitamin Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add all components, except NaHCO3 and
Na2S·9H2O, to distilled/deionized water and bring volume to 1.0L. Gen-
tly heat and bring to boiling under 90% N2 + 10% CO2 . Cool medium to
room temperature while continuing to gas with 90% N2  + 10% CO2 . Add
NaHCO3 and Na2S·9H2O. Adjust pH to 7.5–7.7. Distribute into tubes
under 90% N2 + 10% CO2 using anaerobic techniques. Autoclave for 15
min at 15 psi pressure–121°C.
Use: For the cultivation of anaerobic bacteria that can utilize cinnamic
acid as a carbon source. As a basal medium for the cultivation of
Formivibrio citricus.