Citrate Azide Tween Carbonate Base

[所属分类:培养基配方] [发布时间:2020-4-30] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Citrate Azide Tween Carbonate Base

培养基配方
Composition per liter:
Agar .....................................................................15.0g
Casein enzymic hydrolysate ...............................................15.0g
Sodium citrate............................................................15.0g
Yeast extract..............................................................5.0g
KH2PO4 ....................................................................5.0g
Tween 80...................................................................1.0g
Selective supplement solution............................................10.0mL
pH 7.0 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Selective Supplement Solution:
Composition per 10.0mL:
Na2CO3 ....................................................................1.0g
NaN3 ......................................................................0.2g
2,3,5, Triphenyltetrazolium chloride .....................................0.05g
Preparation of Selective Supplement Solution:  Add compo-
nents to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Preparation of Medium: Add components, except selective sup-
plement solution, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C. Aseptically add selective supplement solution.
Mix thoroughly. Pour into Petri dishes or aseptically distribute into
sterile tubes.
Use: For the identification of enterococci in meat, meat products, dairy
products, and other foodstuffs.