CIN Agar (Yersinia Selective Agar) (Cefsulodin Irgasan ® Novobiocin Agar)

[所属分类:培养基配方] [发布时间:2020-4-30] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CIN Agar
(Yersinia Selective Agar)
(Cefsulodin Irgasan ® Novobiocin Agar)

培养基配方
Composition per liter:
Mannitol...............................................................20.0g
Agar...................................................................12.0g
Pancreatic digest of gelatin...........................................10.0g
Beef extract............................................................5.0g
Peptic digest of animal tissue .........................................5.0g
Sodium pyruvate.........................................................2.0g
Yeast extract...........................................................2.0g
NaCl....................................................................1.0g
Sodium deoxycholate.....................................................0.5g
Neutral Red............................................................0.03g
Cefsulodin............................................................0.015g
Irgasan ® (triclosan)..................................................4.0mg
Novobiocin ............................................................2.5mg
Crystal Violet.........................................................1.0mg
pH 7.4 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components, except cefsulodin and
novobiocin, to distilled/deionized water and bring volume to 1.0L.
Heat, mixing continuously, until boiling. Do not autoclave. Cool to
45°–50°C. Aseptically add cefsulodin and novobiocin. Mix thorough-
ly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the selective isolation and differentiation of Yersinia entero-
colitica from a variety of clinical and nonclinical specimens based on
mannitol fermentation. Yersinia enterocolitica appears as “bull’s eye”
colonies with deep red centers surrounded by a transparent periphery.