Chu’s No. 11 Medium, Modified

[所属分类:培养基配方] [发布时间:2020-4-30] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chu’s No. 11 Medium, Modified

培养基配方
Composition per liter:
NaNO3 ......................................................................1.5g
MgSO4 ·7H2O...............................................................0.08g
Na2SiO3 ·9H2O.............................................................0.06g
CaCl2 ·2H2O...............................................................0.04g
K2HPO4 ·3H2O..............................................................0.04g
Na2CO3 ....................................................................0.02g
Citric acid................................................................6.0mg
Ferric ammonium citrate....................................................6.0mg
EDTA.......................................................................1.0mg
Seawater.................................................................999.0mL
Trace metal solution A5 with cobalt........................................1.0mL
pH 7.5 ± 0.2 at 25°C
Trace Metal Solution A5 with Cobalt:
Composition per liter:
H3BO3 ......................................................................2.86g
MnCl2 ·4H2O................................................................1.81g
Na2MoO4 ·2H2O .............................................................0.39g
ZnSO4 ·7H2O...............................................................0.222g
CuSO4 ·H2O................................................................0.079g
Co(NO3)2 ·6H2O ...........................................................0.049g
Preparation of Trace Metal Solution A5 with Cobalt: Add
components to distilled/deionized water and bring volume to 1.0L. Mix
thoroughly.
Preparation of Medium: Add components to seawater and bring
volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Dis-
tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the isolation and cultivation of cyanobacteria from marine
habitats.