Chu’s No. 10 Medium, Modified

[所属分类:培养基配方] [发布时间:2020-4-30] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chu’s No. 10 Medium, Modified

培养基配方
Composition per liter:
Agar ......................................................................15.0g
Ca(NO3)2 ·4H2O...........................................................0.232g
Na2SiO3 ·5H2O............................................................0.044g
MgSO4 ·7H2O..............................................................0.025g
Na2CO3 ....................................................................0.02g
K2HPO4 ....................................................................0.01g
Citric acid................................................................3.5mg
Ferric citrate.............................................................3.5mg
Metal solution.............................................................1.0mL
Metal Solution:
Composition per liter:
H3BO3 ......................................................................2.4g
MnCl2 ·4H2O................................................................1.4g
ZnCl2 ......................................................................0.4g
CoCl2 ·6H2O ..............................................................0.02g
CuCl2 ·2H2O ..............................................................0.1mg
Preparation of Metal Solution:  Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized wa-
ter and bring volume to 1.0L. Mix thoroughly. Gently heat to boiling. Dis-
tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation and maintenance of Anabaena species and
Plectomena boryanum.