Chromogenic Listeria Agar

[所属分类:培养基配方] [发布时间:2020-4-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chromogenic Listeria Agar

培养基配方
Composition per liter:
Peptone ....................................................................18.5g
LiCl........................................................................15.0g
Agar........................................................................14.0g
NaCl.........................................................................9.5g
Yeast extract................................................................4.0g
Maltose .....................................................................4.0g
Sodium pyruvate .............................................................2.0g
X-glucoside chromogenic mix .................................................0.2g
Differential lecithin solution.............................................40.0mL
Selective supplement solution .............................................20.0mL
pH 7.2 ± 0.2 at 25°C
Source:  This medium is available from Oxoid Unipath.
Differential Lecithin Solution:
Composition per 40.0mL:
Lecithin..................................................................Proprietary
Preparation of Differential Lecithin Solution:  Available as pre-
mixed solution.
Selective Supplement Solution:
Composition per 20.0mL:
Nalidixic acid............................................................26.0mg
Polymyxin B...............................................................10.0mg
Amphotericin..............................................................10.0mg
Ceftazidime................................................................6.0mg
Preparation of Selective Supplement Solution:  Add components
to distilled/deionized water and bring volume to 20.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except differential lec-
ithin solution and selective supplement solution, to distilled/deionized
water and bring volume to 940.0mL. Mix thoroughly. Gently heat
while stirring and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 46°C. Aseptially add differential lecithin solution
and selective supplement solution. Mix thoroughly. Pour into sterile
Petri dishes.
Use:  For the isolation, enumeration, and presumptive identification of
Listeria spp. and Listeria monocytogenes. This selective medium con-
tains the substrate lecithin, which permits differentiation of L. monocy-
togenes and L. Ivanovii from other Listeria species. Differential activ-
ity for all Listeria species is due to the addition of a chromogenic
substrate.