Chromogenic Candida Agar

[所属分类:培养基配方] [发布时间:2020-4-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chromogenic Candida Agar

培养基配方
Composition per liter:
Chromogenic mix .............................................................13.6g
Agar.........................................................................13.6g
Peptone ......................................................................4.0g
Selective supplement solution ..............................................10.0mL
pH 6.0 ± 0.2 at 25°C
Source:  This medium is available from Oxoid Unipath.
Selective Supplement Solution:
Composition per 10.0mL:
Chloramphenicol............................................................500.0mg
Preparation of Selective Supplement Solution:  Add chloram-
phenicol to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0mL. Mix thoroughly. Gently heat while
stirring and bring to boiling. Do not autoclave. Cool to 45°C. Pour into
sterile Petri dishes.
Use:  For the rapid isolation and identification of clinically important
Candida species. The medium incorporates two chromogens that indi-
cate the presence of the target enzymes: X-NAG (5-bromo-4-chloro-3-
indolyl N acetyl ß-D-glucosaminide) detects the activity of hexosamin-
idase. BCIP (5-bromo-6-chloro-3-indolyl phosphate p-toluidine salt)
detects alkaline phosphatase activity. An opaque agent has been incor-
porated into the formulation to improve the color definition on the agar.
The broad-spectrum antibacterial agent chloramphenicol is added to
the agar to inhibit bacterial growth on the plates.