Chromogenic Listeria Agar (ISO)

[所属分类:培养基配方] [发布时间:2020-4-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chromogenic Listeria Agar (ISO)

培养基配方
Composition per liter:
Enzymatic digest of animal tissue ......................................18.0g
Agar ...................................................................12.0g
LiCl....................................................................10.0g
Yeast extract...........................................................10.0g
Enzymatic digest of casein ..............................................6.0g
NaCl.....................................................................5.0g
Na2HPO4 , anhydrous......................................................2.5g
Glucose .................................................................2.0g
Sodium pyruvate .........................................................2.0g
Magnesium glycerophosphage ..............................................1.0g
MgSO4 , anhydrous........................................................0.5g
X-glucoside chromogenic mix ............................................0.05g
L -α-phosphotidylinositol ............................................40.0mL
Selective supplement solution .........................................20.0mL
pH 7.2 ± 0.2 at 25°C
Source:  This medium is available from Oxoid Unipath.
Selective Supplement Solution:
Composition per 20.0mL:
Nalidixic acid.........................................................20.0mg
Ceftazidime............................................................20.0mg
Amphotericin...........................................................10.0mg
Polymyxin B..........................................................76,700 U
Preparation of Selective Supplement Solution:  Add components
to distilled/deionized water and bring volume to 20.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except  L -α-phosphoti-
dylinositol and selective supplement solution, to distilled/deionized
water and bring volume to 940.0mL. Mix thoroughly. Gently heat
while stirring and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 46°C. Aseptially add  L -α-phosphotidylinositol
and selective supplement solution. Mix thoroughly. Pour into sterile
Petri dishes.
Use:  For the isolation, enumeration, and presumptive identification of
Listeria spp. and Listeria monocytogenes. This selective medium con-
tains the substrate lecithin, which permits differentiation of L. monocy-
togenes and L. Ivanovii from other Listeria species.