Brucella Albimi Broth with Agar and 1.5% Sodium Chloride (ATCC Medium 2160)

[所属分类:培养基配方] [发布时间:2020-3-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brucella Albimi Broth
with Agar and 1.5% Sodium Chloride
(ATCC Medium 2160)

培养基配方
Composition per liter:
NaCl..............................................................15.0g
Pancreatic digest of casein.......................................10.0g
Peptic digest of animal tissue ...................................10.0g
Yeast extract......................................................2.0g
Agar...............................................................1.6g
Glucose............................................................1.0g
NaHSO3 ...........................................................0.1g
Horse blood, defibrinated ........................................100.0mL
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components, except horse blood, to
distilled/deionized water and bring volume to 900.0mL. Mix thorough-
ly. Heat gently with frequent mixing. Boil for 1 min. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add
100.0mL of sterile defibrinated horse blood. Mix thoroughly. Asepti-
cally distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Campylobacter nitrofigi-
lis.