Brucella Albimi Broth with Formate and Fumarate

[所属分类:培养基配方] [发布时间:2020-3-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brucella Albimi Broth with Formate and Fumarate

培养基配方
Composition per 1050.0mL:
Pancreatic digest of casein.................................10.0g
Peptic digest of animal tissue .............................10.0g
NaCl........................................................5.0g
Yeast extract...............................................2.0g
Glucose.....................................................1.0g
NaHSO3 .....................................................0.1g
Horse blood, defibrinated ...............................100.0mL
Formate-fumarate solution..................................50.0mL
pH 7.0 ± 0.2 at 25°C
Formate-Fumarate Solution:
Composition per 100.0mL:
Sodium formate ...............................................6.0g
Fumaric acid .................................................6.0g
Preparation of Formate-Fumarate Solution: Add components
to distilled/deionized water and bring volume to 100.0mL. Mix thor-
oughly. Adjust pH to 7.0. Filter sterilize.
Preparation of Medium: Add components, except formate-fumar-
ate solution and horse blood, to distilled/deionized water and bring vol-
ume to 900.0mL. Mix thoroughly. Heat gently with frequent mixing.
Boil for 1 min. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C. Add 100.0mL of sterile defibrinated horse blood. Mix gen-
tly and aseptically distribute into sterile tubes in 5.0mL volumes. Asep-
tically add 0.25mL of formate-fumarate solution to each tube
containing 5.0mL of medium immediately prior to inoculation.
Use: For the cultivation and maintenance of Campylobacter mucosalis.