Brucella Albimi Broth with 0.16% Agar and 1% Glycine (ATCC Medium 2161)

[所属分类:培养基配方] [发布时间:2020-3-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brucella Albimi Broth
with 0.16% Agar and 1% Glycine
(ATCC Medium 2161)

培养基配方
Composition per liter:
Pancreatic digest of casein.................................10.0g
Peptic digest of animal tissue..............................10.0g
Glycine.....................................................10.0g
NaCl.........................................................5.0g
Yeast extract................................................2.0g
Agar ........................................................1.6g
Glucose .....................................................1.0g
NaHSO3 ......................................................0.1g
Horse blood, defibrinated ...................................100.0mL
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components, except horse blood, to
distilled/deionized water and bring volume to 900.0mL. Mix thorough-
ly. Heat gently with frequent mixing. Boil for 1 min. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add
100.0mL of sterile defibrinated horse blood. Mix thoroughly. Asepti-
cally distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Campylobacter species.