Brucella Agar

[所属分类:培养基配方] [发布时间:2020-3-10] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brucella Agar

培养基配方
Composition per liter:
Agar..............................................................15.0g
Pancreatic digest of casein.......................................10.0g
Peptic digest of animal tissue ...................................10.0g
NaCl..............................................................5.0g
Yeast extract......................................................2.0g
Glucose............................................................1.0g
NaHSO3 ............................................................0.1g
Horse blood, defibrinated ......................................100.0mL
pH 7.0 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems and Oxoid Unipath.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 900.0mL. Mix thoroughly. Heat gently with
frequent mixing. Boil for 1 min. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 45°–50°C. Add 100.0mL of sterile defibrinated
horse blood. Mix gently and pour into sterile Petri dishes.
Use: For the cultivation and maintenance of Brucella species. For the
isolation and cultivation of nonfastidious and fastidious microorgan-
isms from a variety of clinical and nonclinical specimens.