Brucella Agar Base Campylobacter Medium

[所属分类:培养基配方] [发布时间:2020-3-10] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brucella Agar Base Campylobacter Medium
Composition per 1100.0mL:
Cycloheximide (actidione).......................................0.05g
Sodium cephazolin..............................................0.015g
Novobiocin......................................................5.0mg
Bacitracin....................................................25,000U
Colistin sulfate..............................................10,000U
Brucella agar base..............................................1.0L
Horse blood, defibrinated ..................................100.0mL
Brucella Agar Base
Composition per liter:
Agar......................................................15.0g
Pancreatic digest of casein...............................10.0g
Peptic digest of animal tissue ...........................10.0g
NaCl.......................................................5.0g
Yeast extract..............................................2.0g
Glucose....................................................1.0g
NaHSO3 ....................................................0.1g
Preparation of Brucella Agar Base: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly.
Optional Supplement:
Composition per 10.0mL:
Sodium pyruvate........................................0.25g
NaHSO3 ................................................0.25g
FeSO4 ·7H2O...........................................0.25g
Preparation of Optional Supplement: Add components to dis-
tilled/deionized water and bring volume to 10.0mL. Filter sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components to 1.0L of prepared
Brucella agar base. Mix thoroughly. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Add 100.0mL of sterile, defibri-
nated horse blood. Addition of 10.0mL of optional supplement will im-
prove growth. Mix thoroughly. Pour into sterile Petri dishes.
Use: For the selective isolation and cultivation of Campylobacter jejuni
from fecal specimens or rectal swabs.