Brucella Agar with 1.0% Glucose

[所属分类:培养基配方] [发布时间:2020-3-10] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brucella Agar with 1.0% Glucose

培养基配方
Composition per liter:
Agar...........................................................15.0g
Peptic digest of animal tissue ................................10.0g
Glucose........................................................10.0g
NaCl............................................................5.0g
Meat extract....................................................5.0g
Sheep blood, defibrinated....................................100.0mL
Horse serum...................................................50.0mL
Vitamin K 1 solution ..........................................1.0mL
pH 7.5 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Vitamin K 1 Solution:
Composition per 20.0mL:
Vitamin K 1 ................................................0.2g
Ethanol, absolute...........................................20.0mL
Preparation of Vitamin K 1 Solution: Add vitamin K 1 to 20.0mL
of ethanol. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except sheep blood,
horse serum, and vitamin K 1 solution, to distilled/deionized water and
bring volume to 849.0mL. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add 100.0mL of sterile sheep blood, 50.0mL of
horse serum, and 1.0mL of sterile vitamin K 1 solution. Mix thoroughly.
Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Brucella species.