Bromthymol Blue Broth

[所属分类:培养基配方] [发布时间:2020-3-10] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bromthymol Blue Broth

培养基配方
Composition per 101.45mL:
Pancreatic digest of casein..................................0.7g
NaCl.........................................................0.5g
Beef extract.................................................0.3g
Yeast extract................................................0.3g
Beef heart, solids from infusion.............................0.2g
Horse serum................................................10.0mL
Bromthymol Blue solution ..................................1.0mL
Ampicillin solution........................................1.0mL
Urea solution..............................................0.25mL
Nystatin solution..........................................0.1mL
Tripeptide solution........................................0.1mL
pH 6.0 ± 0.2 at 25°C
Bromthymol Blue Solution:
Composition per 50.0mL:
Bromthymol Blue.............................................0.2g
NaOH (0.01N solution).....................................32.0mL
Preparation of Bromthymol Blue Solution: Add Bromthymol
Blue to NaOH solution. Mix thoroughly. Bring volume to 50.0mL with
distilled/deionized water. Autoclave for 15 min at 15 psi pressure–
121°C. Store at 25°C .
Ampicillin Solution:
Composition per 10.0mL:
Ampicillin.................................................1.0g
Preparation of Ampicillin Solution:  Add ampicillin to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Urea Solution:
Composition per 100.0mL:
Urea.......................................................10.0g
Preparation of Urea Solution: Add urea to distilled/deionized wa-
ter and bring volume to 100.0mL. Filter sterilize. Store at −20°C.
Nystatin Solution:
Composition per 1.0mL:
Nystatin.................................................. 50,000U
Preparation of Nystatin Solution: Add nystatin to distilled/de-
ionized water and bring volume to 1.0mL. Filter sterilize.
Tripeptide Solution:
Composition per 10.0mL:
Glycyl- L- histidyl- L- lysine acetate........................0.2mg
Preparation of Tripeptide Solution: Add glycyl- L- histidyl- L-
lysine acetate to distilled/deionized water and bring volume to 10.0mL.
Mix thoroughly. Filter sterilize. Store at −20°C.
Preparation of Medium: Add components—except horse serum,
ampicillin solution, urea solution, nystatin solution, and tripeptide so-
lution—to distilled/deionized water and bring volume to 90.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL of
sterile horse serum, 1.0mL of sterile ampicillin solution, 0.25mL of ster-
ile urea solution, 0.1mL of sterile nystatin solution, and 0.1mL of sterile
tripeptide solution. Mix thoroughly. Pour into sterile Petri dishes.
Use: For the cultivation of Ureaplasma species from clinical speci-
mens.