Bromthymol Blue Agar

[所属分类:培养基配方] [发布时间:2020-3-10] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bromthymol Blue Agar

培养基配方
Composition per liter:
Agar ...........................................................11.0g
Peptone.........................................................10.0g
NaCl.............................................................5.0g
Yeast extract....................................................5.0g
Lactose (33% solution).........................................27.0mL
Bromthymol Blue (1% solution)..................................10.0mL
Sodium thiosulfate (50% solution)...............................2.0mL
Glucose (33% solution) .........................................1.2mL
Maranil solution (5% solution)..................................1.0mL
pH 7.7–7.8 at 25°C
Preparation of Medium: Add agar, peptone, NaCl, and yeast extract
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 8.0. Autoclave for 20 min at 15 psi pressure–121°C. Cool
to 45°–50°C. Filter sterilize separately the lactose solution, Bromthymol
Blue solution, sodium thiosulfate solution, glucose solution, and maranil
solution. To the cooled, sterile agar solution aseptically add 27.0mL of
sterile lactose solution, 10.0mL of sterile Bromthymol Blue solution,
2.0mL of sterile sodium thiosulfate solution, 1.2mL of sterile glucose so-
lution, and 1.0mL of sterile maranil solution. Mix thoroughly. Adjust pH
to 7.7–7.8. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the selective isolation and cultivation of members of the
Enterobacteriaceae.