Brooks Agar

[所属分类:培养基配方] [发布时间:2020-3-10] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brooks Agar

培养基配方
Composition per liter:
Agar ..........................................................20.0g
Cornmeal........................................................5.0g
Yeast extract...................................................2.0g
KH2PO4 .........................................................1.5g
Malt extract....................................................1.0g
Pancreatic digest of casein.....................................1.0g
MgSO4 ·7H2O....................................................0.5g
Preparation of Medium: Add cornmeal to distilled/deionized wa-
ter and bring volume to 500.0mL. Gently heat and bring to boiling.
Boil for 30 min. Filter through cotton. Bring volume of filtrate to 1.0L
with distilled/deionized water. Add remaining components. Mix thor-
oughly. Gently heat while stirring and bring to boiling. Autoclave for
15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or dis-
tribute into sterile tubes.
Use: For the cultivation of Aspergillus japonicus, Aspergillus sojae,
and Phytophthora citrophthora.