Bromcresol Purple Milk Yeast Extract with CCG

[所属分类:培养基配方] [发布时间:2020-3-10] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bromcresol Purple Milk Yeast Extract with CCG

培养基配方
Composition per liter:
Milk solution..................................................1.0L
Agar solution...............................................900.0mL
Yeast extract solution.......................................40.0mL
Chloramphenicol solution.....................................10.0mL
Cycloheximide solution.......................................10.0mL
Gentamicin solution...........................................0.8mL
Milk Solution:
Composition per liter:
Skim milk powder..............................................80.0g
Bromcresol Purple solution ...................................2.0mL
Preparation of Milk Solution : Add components to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 8
min at 11 psi pressure–116°C. Cool to 45°–50°C.
Bromcresol Purple Solution:
Composition per 10.0mL:
Bromcresol Purple...............................................0.16g
Ethanol (95% solution).........................................10.0mL
Preparation of Bromcresol Purple Solution: Add Bromcresol
Purple to 10.0mL of ethanol. Mix thoroughly.
Agar Solution:
Composition per 900.0mL:
Agar............................................................30.0g
Preparation of Agar Solution: Add agar to distilled/deionized
water and bring volume to 900.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C.
Yeast Extract Solution:
Composition per 100.0mL:
Yeast extract...................................................40.0g
Preparation of Yeast Extract Solution: Add yeast extract to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize.
Chloramphenicol Solution:
Composition per 10.0mL:
Chloramphenicol..............................................0.1g
Preparation of Chloramphenicol Solution: Add chlorampheni-
col to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Filter sterilize.
Cycloheximide Solution:
Composition per 10.0mL:
Cycloheximide....................................................0.2g
Preparation of Cycloheximide Solution: Add cycloheximide to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Gentamicin Solution:
Composition per 10.0mL:
Gentamicin.....................................................0.5g
Preparation of Gentamicin Solution: Add gentamicin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Aseptically combine the sterile milk so-
lution and sterile agar solution. Aseptically add 40.0mL of sterile yeast
extract solution, 10.0mL of sterile chloramphenicol solution, 10.0mL
of sterile cycloheximide solution, and 0.8mL of sterile gentamicin so-
lution. Mix thoroughly. Aseptically distribute into sterile tubes. Allow
tubes to cool in a slanted position.
Use: For the isolation, cultivation, and differentiation of Trichophyton
verrucosum and Trichophyton schoenleinii.