Bromo Cresol Purple Azide HiVeg Broth

[所属分类:培养基配方] [发布时间:2020-3-10] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bromo Cresol Purple Azide HiVeg Broth

培养基配方
Composition per liter:
Plant hydrolysate .............................................10.0g
Yeast extract..................................................10.0g
NaCl............................................................5.0g
D -Glucose......................................................5.0g
K2HPO4 .........................................................2.7g
KH2PO4 .........................................................2.7g
NaN3 ...........................................................0.5g
Bromo Cresol Purple ..........................................32.0mg
pH 7.0 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
Preparation of Medium: Add components to distilled/deionized
water to 1.0L. Mix thoroughly. Gently heat to boiling. Distribute into
tubes or flasks. Autoclave for 15 min at 10 psi pressure–115°C.
Use: For use in the confirmation test for the presence of fecal strepto-
cocci in water and wastewater.