Bromcresol Purple Dextrose Broth (BCP Broth)

[所属分类:培养基配方] [发布时间:2020-3-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bromcresol Purple Dextrose Broth
(BCP Broth)

培养基配方
Composition per liter:
Glucose......................................................10.0g
Peptone ......................................................5.0g
Beef extract..................................................3.0g
Bromcresol Purple solution ..................................2.0mL
pH 7.0 ± 0.2 at 25°C
Bromcresol Purple Solution:
Composition per 10.0mL:
Bromcresol Purple ......................................................................0.16g
Ethanol (95% solution)............................................................10.0mL
Preparation of Bromcresol Purple Solution: Add Bromcresol
Purple to 10.0mL of ethanol. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
in 12–15mL volumes. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation and differentiation of bacteria based on their
ability to ferment glucose. Bacteria that ferment glucose turn the
medium yellow.