Bromcresol Purple Milk Solids Glucose Agar (BCP MS G Agar)

[所属分类:培养基配方] [发布时间:2020-3-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bromcresol Purple Milk Solids Glucose Agar
(BCP MS G Agar)

培养基配方
Composition per 2.0L:
Skim milk powder...........................................80.0g
Glucose ...................................................40.0g
Agar ......................................................30.0g
Bromcresol Purple solution ................................2.0mL
pH 6.6 ± 0.2 at 25°C
Bromcresol Purple Solution:
Composition per 10.0mL:
Bromcresol Purple .........................................0.16g
Ethanol (95% solution)....................................10.0mL
Preparation of Bromcresol Purple Solution: Add Bromcresol
Purple to 10.0mL of ethanol. Mix thoroughly.
Preparation of Medium: Add skim milk powder and Bromcresol
Purple solution to distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Autoclave for 8 min at 11 psi pressure–116°C. Cool to
45°–50°C. In a separate flask, add glucose to distilled/deionized water
and bring volume to 200.0mL. Mix thoroughly. Autoclave for 8 min at
11 psi pressure–116°C. Cool to 45°–50°C. In a third flask, add agar to
distilled/deionized water and bring volume to 800.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically combine the three
sterile solutions. Mix thoroughly. Aseptically adjust the pH to 6.6 with
sterile 1N HCl. Aseptically distribute into sterile tubes. Allow tubes to
cool in a slanted position.
Use: For the cultivation and differentiation of Trychophyton menta-
grophytes, Trychophyton rubrum, and Microsporum persicolor.