Bromcresol Purple Broth with Sodium Chloride (BAM M26)

[所属分类:培养基配方] [发布时间:2020-3-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bromcresol Purple Broth with Sodium Chloride
(BAM M26)

培养基配方
Composition per liter:
NaCl............................................................25.0g
Peptone ........................................................10.0g
Beef extract.....................................................3.0g
Bromcresol Purple...............................................0.04g
Carbohydrate solution..........................................50.0mL
pH 7.0 ± 0.2 at 25°C
Carbohydrate Solution:
Composition per 50.0mL:
Carbohydrate...................................................25.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 50.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except carbohydrate
solution, to distilled/deionized water and bring volume to 950.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 10 min
at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 50.0mL of
carbohydrate solution. Aseptically distribute into tubes or flasks. Alter-
nately distribute the medium without the carbohydrate solution into
test tubes that contain an inverted Durham tube prior to autoclaving.
Then autoclave for 10 min at 15 psi pressure–121°C. Cool to 25°C, and
aseptically add the carbohydrate solution to each tube to yield a final
carbohydrate concentration of 5%.
Use: For the differentiation of a halophilic Vibrio spp. Bacteria that
ferment the specific carbohydrate turn the medium yellow. When bac-
teria produce gas, the gas is trapped in the Durham tube.