Bromcresol Purple Broth (BAM M26)

[所属分类:培养基配方] [发布时间:2020-3-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bromcresol Purple Broth
(BAM M26)

培养基配方
Composition per liter:
Peptone......................................................10.0g
NaCl..........................................................5.0g
Beef extract..................................................3.0g
Bromcresol Purple ...........................................0.04g
Carbohydrate solution.......................................50.0mL
pH 7.0 ± 0.2 at 25°C
Carbohydrate Solution:
Composition per 50.0mL:
Carbohydrate.................................................25.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 50.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except carbohydrate
solution, to distilled/deionized water and bring volume to 950.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 10 min
at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 50.0mL of
carbohydrate solution. Aseptically distribute into tubes or flasks. Alter-
nately distribute the medium without the carbohydrate solution into
test tubes that contain an inverted Durham tube prior to autoclaving.
Then autoclave for 10 min at 15 psi pressure–121°. Cool to 25°C, and
aseptically add the carbohydrate solution to each tube to yield a final
carbohydrate concentration of 5%.
Use: For the differentiation of a variety of microorganisms based on
their fermentation of specific carbohydrates. Bacteria that ferment the
specific carbohydrate turn the medium yellow. When bacteria produce
gas, the gas is trapped in the Durham tube.