Bromcresol Purple Broth

[所属分类:培养基配方] [发布时间:2020-3-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bromcresol Purple Broth

培养基配方
Composition per liter:
Peptone.....................................................10.0g
NaCl.........................................................5.0g
Beef extract.................................................3.0g
Bromcresol Purple ..........................................0.04g
Carbohydrate solution......................................10.0mL
pH 7.0 ± 0.2 at 25°C
Carbohydrate Solution:
Composition per 10.0mL:
Carbohydrate.................................................5.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into test tubes that contain an inverted Durham
tube. Autoclave for 10 min at 15 psi pressure–121°C.
Use: For the differentiation of a variety of microorganisms based on
their fermentation of specific carbohydrates. Bacteria that ferment the
specific carbohydrate turn the medium yellow. When bacteria produce
gas, the gas is trapped in the Durham tube.