Bacillus Xylose Salts

[所属分类:培养基配方] [发布时间:2019-12-3] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bacillus Xylose Salts

培养基配方
Composition per liter:
Yeast extract..................................................5.0g
Xylose.........................................................5.0g
NaCl...........................................................1.0g
NH4Cl .........................................................1.0g
KH2PO4 ........................................................0.5g
MgSO4 ·7H2O...................................................0.5g
CaCl2 ·2H2O .................................................0.05g
Trace mineral solution.......................................10.0mL
Vitamin solution.............................................10.0mL
pH 4.0 ± 0.2 at 25°C
Trace Mineral Solution:
Composition per liter:
CoCl2 ·6H2O...................................................0.2g
FeSO4 ·7H2O..................................................0.13g
ZnCl2 ·2H2O ..................................................0.1g
MnCl2 ·4H2O ..................................................0.1g
CaCl2 ·2H2O.................................................20.0mg
Na2SeO3 .....................................................20.0mg
Na2WO4 ·2H2O................................................20.0mg
NaMoO4 ·2H2O.................................................1.0mg
H3BO3 ........................................................0.5mg
CuSO4 ·5H2O..................................................0.4mg
KI.............................................................0.1mg
Preparation of Trace Mineral Solution: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Vitamin Solution:
Composition per liter:
Pyridoxine·HCl..............................................10.0mg
Thiamine·HCl ................................................5.0mg
Riboflavin ...................................................5.0mg
Nicotinic acid................................................5.0mg
Calcium pantothenate .........................................5.0mg
p-Aminobenzoic acid...........................................5.0mg
Thioctic acid ................................................5.0mg
Biotin .......................................................2.0mg
Folic acid....................................................2.0mg
Cyanocobalamin ...............................................0.1mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Adjust pH of medium to 4.0. Distribute into tubes or flasks.
Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation and maintenance of Bacillus species that can
utilize xylose as a carbon source.