Bacillus tusciae Medium

[所属分类:培养基配方] [发布时间:2019-12-3] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bacillus tusciae Medium

培养基配方
Composition per liter:
Agar ......................................................15.0g
Na2HPO4 ·2H2O..............................................2.9g
KH2PO4 .....................................................2.3g
NH4Cl ......................................................1.0g
MgSO4 ·7H2O................................................0.5g
NaHCO3 .....................................................0.5g
Fe(NH4) citrate............................................0.05g
CaCl2 ·2H2O...............................................0.01g
MnSO4 ·H2O................................................0.01g
Ferric ammonium citrate solution..........................20.0mL
Carbon source.............................................10.0mL
Trace elements solution SL-6...............................5.0mL
pH 4.0 ± 0.2 at 25°C
Ferric Ammonium Citrate Solution:
Composition per 20.0mL:
Ferric ammonium citrate....................................0.05g
Preparation of Ferric Ammonium Citrate Solution:  Add fer-
ric ammonium citrate to distilled/deionized water and bring volume to
20.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C.
Carbon Source:
Composition per 10.0mL:
Carbohydrate..................................................2.0g
Organic acid (alternate) .....................................1.0g
Preparation of Carbon Source:  Add either carbohydrate or or-
ganic acid to distilled/deionized water and bring volume to 10.0mL.
Mix thoroughly. Filter sterilize.
Trace Elements Solution SL-6:
Composition per liter:
MnCl2 ·4H2O .................................................0.5g
H3BO3 .........................................................0.3g
CoCl2 ·6H2O...................................................0.2g
ZnSO4 ·7H2O...................................................0.1g
Na2MoO4 ·2H2O................................................0.03g
NiCl2 ·6H2O..................................................0.02g
CuCl2 ·2H2O..................................................0.01g
Preparation of Trace Elements Solution SL-6:  Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except ferric ammoni-
um citrate solution, trace elements solution SL-6, and carbon source, to
distilled/deionized water and bring volume to 965.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Adjust pH to 4.0. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add
20.0mL of sterile ferric ammonium citrate solution, 10.0mL of sterile
carbon source, and 5.0mL of sterile trace elements solution SL-6. Mix
thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the heterotrophic growth of Bacillus tusciae.