Bacterial Cell Agar (BCA)

[所属分类:培养基配方] [发布时间:2019-12-3] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bacterial Cell Agar
(BCA)

培养基配方
Composition per liter:
Tryptose .............................................17.36g
Agar ..................................................15.0g
NaCl...................................................8.68g
Beef extract............................................5,2g
Yeast extract...........................................1.7g
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add components, except agar, to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to 30°C. Inoculate
with a culture of Aeromonas hydrophila. Incubate with shaking at 30°C
for 72 hr. Centrifuge culture in 40.0mL volumes at 10,000 × g for 10
min. Wash the cells four times in sterile 0.85% saline. Resuspend the
cell pellet in 25.0mL of distilled/deionized water. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C. In a separate flask, add
15.0g of agar to 1.0L of distilled/deionized water. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 45°–50°C. Aseptically combine 25.0mL of
washed cells and 250.0mL of cooled, sterile agar solution. Mix thor-
oughly. Pour into sterile Petri dishes.
Use: For the cultivation of freshwater Myxobacterium species.