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Oxidation-Fermentation Medium, King’s (King’s OF Medium)

[所属分类:培养基配方] [发布时间:2021-12-8] [发布人:网站管理员2] [阅读次数:] [返回]

Oxidation-Fermentation Medium, King’s

(King’s OF Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Base:

Agar ....................................................................3.0g

Pancreatic digest of casein..................................2.0g

Carbohydrate solution................................. 100.0mL

Phenol Red (1.5% solution) ............................ 2.0mL

pH to 7.3 ± 0.2

Carbohydrate Solution:

Composition per 100.0mL:

Carbohydrate.....................................................10.0g

Preparation of Carbohydrate Solution: Add

carbohydrate to distilled/deionized water and bring

volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept carbohydrate solution, to distilled/deionized wa

ter and bring volume to 900.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add 100.0mL of sterile carbohydrate so

lution. Mix thoroughly. Pour into sterile Petri dishes

or distribute into sterile tubes.

Use: For differentiating bacteria based upon deter

mining the oxidative and fermentative metabolism of

carbohydrates. Bacteria that ferment the carbohy

drate turn the medium yellow.